Terra Bioforge’s technology enables rapid characterization of biosynthetic gene clusters and their corresponding natural products. In a recent paper out of the Beemelmanns Lab at Saarland University and published in Communications Chemistry, the authors surveyed the chemical diversity of macrolactam natural products. To facilitate characterization of these compounds, the researchers turned to Terra Bioforge for our DNAtrap™ cloning method to capture the intact 105-kb cluster from the Amycolatopsis native host. The captured gene cluster was heterologously expressed in Streptomyces confirming that the pathway encodes for production of the ciromicin macrolactam.
Read the paper “Genome mining for macrolactam-encoding gene clusters allowed for the network-guided isolation of β-amino acid-containing cyclic derivatives and heterologous production of ciromicin A” here: https://www.nature.com/articles/s42004-023-01034-w